SAA Turbidimetric Immunoassay
New SAA Turbidimetric Immunoassay (TIA) - November 2025
The benefits of testing for Serum Amyloid A (SAA) as an indicator of the health status of an animal - as well as a performance indicator in horses - are well documented and have been discussed in other areas on the website.
SAA has become an extremely important parameter as a diagnostic tool for the clinician in recent years. This has resulted in an increasing demand for high numbers of samples to be analysed, which led to Tridelta developing an accurate, reliable, and high throughput TIA easily adapted for a broad range of automated clinical chemistry analyzers in a laboratory environment.
Tridelta's SAA TIA has the same levels of performance and accuracy as our well-known and established Multispecies SAA ELISA. (Cat. Number: TP-802)
The Tridelta SAA Turbidimetric Immunoassay is now available.
The SAA TIA utilises the same monoclonal antibody as used in our PHASE Multispecies SAA ELISA, developed and optimised for veterinary samples, and is in a modular format consisting of three separate components for maximum efficiency and economy:
SAA Turbidimetric Immunoassay Reagent kit (Cat. # TE-501-RGT)
SAA Turbidimetric Immunoassay Calibrator set - horses, dogs, cows, others (Cat. # TE-501-CAL)
SAA Turbidimetric Immunoassay Calibrator set - cats and pigs (Cat. # TE-501-FCAL)
SAA Turbidimetric Immunoassay Control set (Cat. # TE-501-CON)
A prime requirement of the TIA is that it correlates with the SAA ELISA which has been an effective “gold standard” in this field for many years.
The following graph is the result of testing a large number of equine serum samples (n = 71) covering a very broad range of SAA concentrations in both the new Tridelta SAA Turbidimetric Immunoassay and the Tridelta Multispecies SAA ELISA Assay (TP-802).
The regression equation (using an intercept of zero) and r2 values were calculated to be y=0.9806x and 0.91 respectively, showing that this method can be used reliably to calculate SAA concentrations in equine serum.
Accuracy and reliability are key parameters in using any assay and it is of particular importance that factors such as the “hook effect” are minimised. The assay was rigorously tested in this respect and no hook effect was observed when a sample pre-dilution of 1:10 was carried out, followed by a reflex dilution of 1:5, up to and including extreme SAA concentrations of 12.2 mg/ml.
Stability
The calibration curve, when established on the instrument, is stable for a period of up to 3 weeks. Daily controls should be run and if the controls return within the expected range there is no need to re-calibrate – however, if controls are out of range, it is recommended that the calibration curve be re-run.
Analytical Sensitivity (Equine)
The sensitivity of the assay, when used on the Cobas Mira Plus is determined as 2.4ug/ml
Measuring Range (Equine)
The upper limit of the assay is dependent on the highest calibration point value of the calibration curve: 300ug/ml. When a sample pre-dilution of 1:10 is used this is extended to 3000ug/ml. For the extremes of measuring, a further reflex dilution of 1:5 can be used which extends the measuring range to 15mg/ml.
Precision (Equine)
Inter and Intra Assay Precision were measured with the following results:
Storage and Use
The Reagent kit is stored and used at 4C. The Control kit is stored at 4C in the short term and at -20C for longer periods. The Calibrator kit is stored at -20C. Consult the individual pack inserts for further information.
The New SAA Turbidimetric Immunoassay is now available to order directly from Tridelta or via your local Tridelta Distributor.
